Microbes can be a solution to many problems that world is facing today. one burning problem is increasing environmental pollution caused by indiscriminate use of fossil fuels. Searching for alternative fuel source that is renewable as well as environmentally friendly is taking momentum in past few years. Bioenergy; energy from biomass especially plant, has potential to fulfull world energy demand, and microbes as converter of biomass to useful products like bioethanol, biohydrogen, biomethane which can be used for energy production. MDBioenergy is a database of potential microbial species that can be used to produce bioenergy. The database is desinged on relational database concept, and it consists of species taxonomy, its genes, proteins and enzymes involved in energy conversion pathway. The database will be useful to all who are working on microbial strain improvement for bioenergy production.